Light quality affects morphology and polysaccharide yield and composition of Gelidium sesquipedale (Rhodophyceae) |
| |
Authors: | Raquel Carmona Juan J Vergara Marc Lahaye F X Niell |
| |
Institution: | (1) Departamento de Ecología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain;(2) Departamento de Ecología, Facultad de Ciencias del Mar, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain;(3) Institut National de la Recherche Agronomique, Unite de Recherche sur les Polysaccharides, leurs Organisations et Interactions, BP 71627, 44316 Nantes, France |
| |
Abstract: | Morphology and polysaccharide characterization of Gelidium sesquipedale (Clem.) Bornet et Thuret were studied in cultures
grown under various light qualities. White light (WL), blue light (BL) and red light (RL) (all at photon fluence rate of 40
μmol m-2 s-1) were used for the study of morphological characteristics, and in addition yellow light (YL) for polysaccharide
characterization. RL and BL induced a proliferating growth, which resulted in bushy plants under RL. Cortical cells of BL-grown
plants were smaller and presented a higher density per unit area, whereas those of WL- and RL-grown alga were larger. Medullary
cells followed the inverse pattern. Light quality also affected polysaccharide yield and composition, with the yield being
higher under BL, RL or YL than WL. Most of the polysaccharide was extracted in distilled water at 100 °C, while a low amount
was solubilized at 22 °C and 120 °C. Extracts from BL-grown alga presented the highest galactan content. The starch concentration
was lower in extracts from RL-, BL- and YL-cultivated alga than in those from the initial plants. The degree of substitution
with methoxyl groups and precursor was very low in all the agar fractions, but fractions extracted from BL- and WL-grown alga
were more substituted by precursor. The highest sulfate content was reached under BL (about 9% w/w) and the highest 2-O-methyl-3,6-anhydro-L-galactose
and 6-O-methyl-D-galactose content were found in extracts from alga grown under YL.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | Gelidium sesquipedale light quality branch agar starch |
本文献已被 SpringerLink 等数据库收录! |
|