Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: involvement of pertussis toxin-sensitive G-protein coupled receptor |
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Authors: | Park Kyoung Sun Lee Ha Young Lee Sun Young Kim Mi-Kyoung Kim Sang Doo Kim Jung Mo Yun Jeanho Im Dong-Soon Bae Yoe-Sik |
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Institution: | Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Republic of Korea. |
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Abstract: | We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration (Ca(2+)](i)) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited Ca(2+)](i) increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. |
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Keywords: | LPA lysophosphatidic acid LPS lysophosphatidylserine LPG lysophosphatidylglycerol GPCR G-protein coupled receptor LPE lysophosphatidylethanolamine fura-2/AM fura-2 pentaacetoxymethylester PTX pertussis toxin ERK extracellular signal regulated protein kinase [Ca2+]i intracellular calcium concentration PLC phospholipase C NF-κB nuclear factor kappa B |
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