Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of <Emphasis Type="Italic">Areca catechu</Emphasis> L. (Arecaceae) |
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Authors: | Hsiang-Chih?Wang Jen-Tsung?Chen Shieh-Ping?Wu Mei-Chun?Lin Email author" target="_blank">Wei-Chin?ChangEmail author |
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Institution: | (1) Institute of Botany, Academia Sinica, 115 Taipei Taiwan, Republic of China |
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Abstract: | Summary An in vitro culture procedure was established for somatic embryogenesis and plant regeneration from callus cultures of the important
palm ‘betel nut’ (Areca catechu L.). Segments of zygotic embryos of Areca catechu L. were cultured on Murashige and Skoog basal medium supplemented with dicamba (9.05, 18.1, 27.15, and 36.2 μM). After 7–8 wk in darkness, wounded regions of explants formed callus with yellow, soft, glutinous structures. Proliferation
and maintenance of callus was on the same dicamba-containing medium. With regular subculture every 8 wk, the callus showed
pale yellow, compact and nodular structures. During subculture, somatic embryos were formed spontaneously from nodular callus
tissues within 2–4 mo. The embryos developed into plantlets after 10 wk of culture on basal medium free of plant growth regulators.
After subculturing every month for 3 mo., the plantlets were transferred to containers for acclimatization in the greenhouse.
The survival rate was 24%. |
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Keywords: | betel nut palm somatic embryo |
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