基于rDNA序列分析的湖北克鲁维酵母系统发育地位探讨 |
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引用本文: | 赵建花,贾建华,白逢彦.基于rDNA序列分析的湖北克鲁维酵母系统发育地位探讨[J].菌物学报,2004,23(1):28-32. |
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作者姓名: | 赵建花 贾建华 白逢彦 |
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作者单位: | 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100080 |
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基金项目: | Supported by grants KSCX2-SW-101C from the Chinese Academy of Sciences and 2001AA227131 of the 863 program from the Ministry of Science and Technology,China. |
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摘 要: | 国内于20世纪90年代初曾描述过两个克鲁维酵母新种:中国克鲁维酵母(Kluyveromyces sinensis M. X. Li et al.)和湖北克鲁维酵母(Kluyveromyces hubeiensis M. X. Li et al.),二者均分自湖北神农架自然保护区。前者已被国际酵母菌分类学研究者接受和承认,而后者却一直被忽视。本文根据小亚基(18S) rRNA基因、大亚基(26S) rRNA基因D1/D2区和转录间区(ITS)序列分析,对K. hubeiensis进行了分子系统学研究。结果显示,K. hubeiensis代表一个具有充分分子系统学数据支持的独立种,该种与Saccharomyces spencerorum和Kluyveromyces lodderae形成一个高支持率的分枝,且与前者更近缘。本研究还显示,K. sinensis与Saccharomyces naganishii具有很近的亲缘关系。鉴于目前仅依靠序列分析对Kluyveromyces和Saccharomyces及其它相关属进行调整尚不现实,故建议仍维持这两个属的传统概念,并继续使用原种名。
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关 键 词: | 克鲁维酵母属 酿酒酵母属 中国克鲁维酵母 分子系统学 |
PHYLOGENETIC POSITION OF KLUYVEROMYCES HUBEIENSIS AS REVEALED FROM RDNA SEQUENCE ANALYSIS |
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Authors: | Abstract |
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Abstract: | The phylogenetic position of Kluyveromyces hubeiensis was investigated on the basis of 18S rDNA, internal transcribed spacer (ITS) region (including 5.8S rDNA) and 26S rDNA D1/D2 domain sequence analyses. The result indicated that K. hubeiensis is a distinct species closely related to Saccharomyces spencerorum and Kluyveromyces lodderae, with a closer relationship to the former. The close relationship between Kluyveromyces sinensis and Saccharomyces naganishii was also showed by the rDNA sequence analyses. |
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Keywords: | Kluyveromyces sinensis Saccharomyces molecular phylogeny |
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