Characterization of a strawberry late-expressed and fruit-specific peptide methionine sulphoxide reductase |
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Authors: | Andrés Pedraza Lopez Rosario Blanco Portales Juan Antonio López-Ráez Nieves Medina-Escobar Juan Muñoz Blanco Antonio Rodríguez Franco |
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Institution: | Dpto. Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus Universitario de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain; Present address: Dpto. Biología y Ciencias del Medio Ambiente, Facultad de Ciencias Experimentales, Universidad SEK, Cardenal Zúñiga 12, 40003 Segovia, Spain |
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Abstract: | We have cloned and characterized a cDNA clone, called Fapmsr , coding for a putative peptide methionine sulphoxide Met(O)] reductase (PMSR, EC 1.8.4.6) from strawberry fruits ( Fragaria x ananassa ). This gene is involved in the repair of inactive peptides and proteins caused for the oxidation of methionine residues to Met(O). Expression of the Fapmsr was only detected in the receptacles of red mature fruits and not in young or immature fruits nor in other plant tissues such as flowers, leaves, runners, roots or achenes. Expression of the Fapmsr gene was activated in green immature fruits when achenes were removed from receptacles, and this was prevented by the application of exogenous auxins such as naphthaleneacetic acid. The enzyme produced and purified by cloning the strawberry cDNA in frame with the C-terminal sequence of the glutathione S-transferase gene can reduce free Met(O) to methionine as analysed by reverse phase high performance liquid chromatography. We have also set up a PMSR protection assay that demonstrates that this enzyme can protect in vivo against the damage produced by the addition of H2O2. |
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