Free Radicals in Mercury-Resistant Bacteria Indicate a Novel Metabolic Pathway |
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Authors: | Ostrovsky D N Diomina G R Biniukov V I Shashkov A S Schloter M |
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Institution: | (1) Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia;(2) Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, Moscow, 117334, Russia;(3) Institute of Soil Ecology, Oberschleissheim, Germany |
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Abstract: | A mercury resistant-soil bacterium P.10.15, identified as a close relative of Pseudomonas veronii, was shown to accumulate a specific compound in the stationary phase of growth. This compound is converted to a long-lived free radical under oxidizing conditions, as registered by its EPR signal at room temperature. The compound was purified by ion-exchange and gel-filtration chromatography and identified by mass spectroscopy, 2D NMR, and EPR as a trisaccharide -D-GlcpNOH,CH3-(16)--D-Glcp-(11)--D-Glcp, or, in other words, as 6-O-(2-deoxy-2-{N-methyl}hydroxylamino--D-glucopyranosyl)---trehalose, previously discovered in Micrococcus luteus (lysodeikticus) and named lysodektose. It is suggested that the compound is a novel intermediate of a previously unknown basic metabolic pathway of trehalose transformation in bacteria, a potential target for antibacterial drug development. |
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Keywords: | mercury trehalose Pseudomonas radicals |
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