Stabilization of Cortical Microtubules in Maize Mesocotyl Cells by Gibberellin A3 |
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Authors: | Hamada Hajime; Mita Takashi; Shibaoka Hiroh |
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Institution: | 1Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka, 560 Japan
2Department of Food and Nutritional Sciences, University of Shizuoka Shizuoka, 422 Japan |
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Abstract: | Effects of GA3 on the stability of cortical microtubules (MTs)were studied in mesocotyl cells of etiolated maize seedlings.Propyzamide, an MT-disrupting agent specific for plant tubulin,disrupted cortical MTs in cells in the upper regions of mesocotylsof GA3-untreated seedlings and caused swelling of the cells.GA3 prevented propyzamide from disrupting MTs and from causingsuch swelling. Chilling of mesocotyls at 4°C for 60 minbrought about the disruption of cortical MTs in cells in theupper regions of mesocotyls of GA3-untreated seedlings, butnot in cells in corresponding regions of GA3-treated seedlings,suggesting that treatment with GA3 increased the stability ofcortical MTs in maize mesocotyl cells. Cortical MTs in protoplastsisolated from mesocotyls of GA3-untreated seedlings failed towithstand chilling at 0°C for 90 min, while those in protoplastsisolated from mesocotyls of GA3-treated seedlings withstoodchilling successfully. It appears that the cell wall is notinvolved in the stabilization of cortical MTs by GA3 in maizemesocotyl cells. (Received July 6, 1993; Accepted November 29, 1993) |
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