Metabolism and activation of benzo[a]pyrene by mouse and rat skin in short-term organ culture and in vivo |
| |
Authors: | A Weston P L Grover P Sims |
| |
Institution: | Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, Fulham Road, London SW3 6JB U.K. |
| |
Abstract: | The metabolic activation of BP was examined in mouse and rat skin in vivo and in short-term organ culture. In mouse skin, larger quantities of ether- and water-soluble metabolites were formed and more BP became bound covalently to DNA and protein than in rat skin. Qualitative differences in the formation of dihydrodiol metabolites and of BP-deoxyribonucleoside adducts between mouse and rat skin were also observed. Organ culture techniques may not provide a true model of metabolic activation in vivo because it was found that the covalent binding of BP to DNA and protein was reduced in skin maintained in culture despite an accumulation of dihydrodiol and other ether-soluble metabolites. In addition, the proportions of the syn- and anti-isomers of BP-7,8-diol 9,10-oxide involved in the formation of adducts with deoxyguanosine differed between skin treated in organ culture and in vivo. |
| |
Keywords: | Benzo[a]pyrene DNA adducts Dihydrodiols Skin BP 9-OHBP BP-4 5-diol BP-7 8-diol BP-9 10-diol BP-11 12-diol 9-OHBP 4 5-oxide HPLC high pressure liquid chromatography TLC thin-layer chromatography |
本文献已被 ScienceDirect 等数据库收录! |