Primate repetitive DNAs: evidence for new satellite DNAs and similarities in non-satellite repetitive DNA sequence properties |
| |
Authors: | Kenneth A. Marx Ian F. Purdom Kenneth W. Jones |
| |
Affiliation: | (1) Institute of Animal Genetics, University of Edinburgh, EH9 3JN Edinburgh, Scotland, UK;(2) Present address: Department of Chemistry, Dartmouth College, 03755 Hanover, New Hampshire, USA |
| |
Abstract: | Repetitious DNA sequences have been isolated from a number of the primates in both Suborders Anthropoidea and Prosimii by hydroxyapatite chromatography at a C0t of 10. In addition to finding previously unreported possible AT-rich satellite DNAs in Orangutan, Gibbon, Rhesus and Slow Loris a clear similarity to human DNA was found in the non-satellite repetitious DNA sequence properties of the primates in the Suborder Anthropoidea. This is based on the presence of the hydroxyapatite isolated 1.703 and 1.714 g/cm3 DNA families in CsCl gradients in the analytical ultracentrifuge following renaturation and extensive DNA hyperpolymer network formation. Within the superfamily Hominoidea the amount of the 1.714 g/cm3 DNA family was greater than that of the 1.703 g/cm3 DNA family while the reverse situation was true within the Superfamily Cercopithecoidea. The orangutan 1.703 and 1.714 g/cm3 DNA families were shown to exhibit the same differential reassociation behavior demonstrated previously in human DNA (Marx et al., 1976a). These data are interpreted as preliminary evidence for a similar sequence organization in the Order Primates Suborder Anthropoidea. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|