Effects of Temperature on Arachidonic Acid-Induced Cellular Edema and Membrane Perturbation in Rat Brain Cortical Slices |
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Authors: | Pak Hoo Chan Robert A. Fishman Sylvia Chen Susan Chew |
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Affiliation: | Brain Edema Clinical Research Center, Department of Neurology, University of California, School of Medicine, San Francisco, California, U.S.A. |
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Abstract: | The effects of temperature on arachidonic acid-induced cellular edema in the first cortical brain slices of rats were studied. Incubation of the cortical slice in arachidonic acid at 37 degrees C induced cellular swelling, and increased intracellular Na+ and lactic acid contents concomitant with decreased intracellular K+. When the incubation temperature was reduced these changes were reduced in severity. The uptake of [3H]arachidonic acid in cortical slices was temperature-dependent. The incorporation of [3H]arachidonic acid into various lipid fractions was further studied by HPLC. The majority of [3H]arachidonic acid was incorporated into triacylglycerol and phosphatidylinositol (PI), but the incorporation of [3H]arachidonic acid into PI was temperature-dependent, unlike that into other phospholipids and neutrolipids. Further, cortical (Na+ + K+)-ATPase activity was inhibited whereas its subunit K+-activated p-nitrophenyl-phosphatase was activated by arachidonic acid at various incubation temperatures. The effects of arachidonic acid on these enzymes is similar to that of thimerosal, a lipid removal agent. These data suggest that both temperature and arachidonic acid play an important role in the development of cellular edema associated with membrane perturbation and inactivation of (Na+ + K+)-ATPase activity. |
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Keywords: | Arachidonic acid Temperature perturbation Cellular edema (Na++ K+)-ATPase Membrane phospholipids |
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