Synaptic degeneration and remodelling after fast kindling of the olfactory bulb |
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Authors: | David P D Woldbye Tom G Bolwig Jørn Kragh Ole Steen Jørgensen |
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Institution: | (1) Laboratory for Experimental Neuropsychiatry, Department of Psychiatry, The National University Hospital: Rigshospitalet-6234, DK-2100 Copenhagen, Denmark;(2) Laboratory for Experimental Neuropsychiatry, Department of Pharmacology, University of Copenhagen, Copenhagen, Denmark, Denmark;(3) Department of Pharmacology, The National University Hospital: Rigshospitalet-6102, Copenhagen, Denmark |
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Abstract: | Kindling of the olfactory bulb using a novel fast protocol (within 24 h) was studied in rats. In target brain regions, the
effects of kindling were measured on the concentration of glial fibrillary acidic protein (GFAP) by dot-blot and on the concentrations
of neural cell adhesion molecule (NCAM) and the 25 kDa synaptosomal associated protein of the D3 immunoprecipitate (D3(SNAP-25))
by crossed immunoelectrophoresis. Bilateral increases in the levels of GFAP, indicating activation of astrocytes, were detected
in primary olfactory cortical projection areas, including the piriform cortex, and also in the basolateral amygdala and dentate
gyrus, suggesting that these regions may be functionally altered during the kindling process. In the piriform cortex and dentate
gyrus increased NCAM/D3(SNAP-25) ratios found ipsilaterally at seven days after kindling probably reflect an elevated rate
of synaptic remodelling. At this time, however, an overall pattern of ipsilateral decreases in the synaptic marker proteins
NCAM and D3(SNAP-25) indicated that this remodelling occurred on a background of synaptic degeneration. These results confirm
previous studies showing that kindling is associated with synaptic remodelling and neuronal degeneration in the hippocampal
formation and extends the area of plasticity to include the piriform cortex which is believed to be central to the kindling
process. |
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Keywords: | Fast kindling seizures synaptic remodelling synaptic degeneration astrocytic activation GFAP neural cell adhesion molecule NCAM SNAP-25 |
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