Phenylalanine Ammonia-Lyase Activity in Soybean Roots Extract Measured by Reverse-Phase High Performance Liquid Chromatography |
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| Authors: | M L L Ferrarese J D Rodrigues O Ferrarese-Filho |
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| Institution: | 2Department of Biochemistry, University of Maringá, Av. Colombo 5790, Maringá, PR, Brazil;1Department of Botany, IB, Unesp, 18618-000, Botucatu, SP, Brazil |
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| Abstract: | Abstract: The high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity in soybean ( Glycine max L. Merril cv. BR16) roots. t -Cinnamate, the catalytic product of the PAL reaction was quantified at 275nm by isocratic elution with methanol: water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non-purified extract. |
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| Keywords: | t-Cinnamate HPLC PAL soybean |
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