alpha-D-Glcp-(1<-->1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase |
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| Authors: | Fransen C T Van Laere K M van Wijk A A Brüll L P Dignum M Thomas-Oates J E Haverkamp J Schols H A Voragen A G Kamerling J P Vliegenthart J F |
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| Affiliation: | Department of Bio-Organic Chemistry, Utrecht University, Netherlands. |
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| Abstract: | A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)]n-alpha-D-Glcp- (1<-->1)-beta-D-Galp[-(4<--1)-beta-D-Galp]m, with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp- (1<-->1)-beta-D-Galp. |
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