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Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter. |
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| Authors: | Margaret L Palmer Mary Alice Raker Poindexter J Kennedy Jon W Young Wayne M Barnes Raymond L Rodriguez and Harry F Noller |
| Institution: | (1) Thimann Laboratories, University of California at Santa Cruz, 95064 Santa Cruz, CA, USA;(2) Present address: Dept. of Genetics, Univ. of California at Davis, 95616 Davis, CA, USA;(3) Present address: Basel Institute for Immunology, Grenzacher-strasse 487, Basel, Switzerland;(4) Present address: Dept. of Biol. Chem, Washington Univ. School of Medicine, 63110 St. Louis, Missouri, USA |
| Abstract: | Summary Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA. |
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