deepBlink: threshold-independent detection and localization of diffraction-limited spots |
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Authors: | Bastian Th Eichenberger YinXiu Zhan Markus Rempfler Luca Giorgetti Jeffrey
A Chao |
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Institution: | Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland;University of Basel, 4003 Basel, Switzerland |
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Abstract: | Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and experimental data. |
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