A gastrointestinal nematode in pregnant and lactating mice alters maternal and neonatal microbiomes |
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| Affiliation: | 1. Institute of Parasitology, McGill University (Macdonald Campus), 21 111 Lakeshore Road, Ste-Anne-de-Bellevue, Québec H9X 3V9, Canada;2. School of Human Nutrition, McGill University (Macdonald Campus), 21 111 Lakeshore Road, Ste-Anne-de-Bellevue, Québec H9X 3V9, Canada;1. Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, China;2. Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, China;3. Centre for Emerging, Endemic and Exotic Diseases (CEEED), Department of Pathology and Population Sciences, Royal Veterinary College, University of London, London, United Kingdom;1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russia;2. Institute of Solid State Chemistry and Mechanochemistry, SB RAS, Novosibirsk, Russia;3. AO Vector-Best, Novosibirsk, Russia;4. Novosibirsk State University, Novosibirsk, Russia;1. Center for Global Health and Diseases, Case Western Reserve University, Biomedical Research Building, 2109 Adelbert Rd., Cleveland, OH 44106, USA;2. University of New Mexico, Department of Anthropology, Albuquerque, 1 University of New Mexico, NM 87131, USA;3. Bahiana School of Medicine and Public Health, Av. Silveira Martins, n° 3386, Salvador, Bahia 41150-100, Brazil;4. Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121 Brotas, Salvador, Bahia 40296-710, Brazil;5. School of Medicine, Federal University of Bahia, Salvador, Bahia, Brazil;6. Yale School of Public Health, Yale University, New Haven, CT, USA;7. Department of Tropical Medicine, Tulane School of Public Health and Tropical Medicine, Tidewater Building, 1440 Canal Street, New Orleans, LA 70112, USA;1. Mitrani Department of Desert Ecology, Swiss Institute of Dryland Environmental and Energy Research, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, Midreshet Ben-Gurion, Israel;2. Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, South Africa;3. Department of Conservation Ecology and Entomology, Stellenbosch University, Matieland, South Africa |
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| Abstract: | The maternal microbiome is understood to be the principal source of the neonatal microbiome but the consequences of intestinal nematodes on pregnant and lactating mothers and implications for the neonatal microbiome are unknown. Using pregnant CD1 mice infected with Heligmosomoides bakeri, we investigated the microbiomes in maternal tissues (intestine, vagina, and milk) and in the neonatal stomach using MiSeq sequencing of bacterial 16S rRNA genes. Our first hypothesis was that maternal nematode infection altered the maternal intestinal, vaginal, and milk microbiomes and associated metabolic pathways. Maternal nematode infection was associated with increased beta-diversity and abundance of fermenting bacteria as well as Lactobacillus in the maternal caecum 2 days after parturition, together with down-regulated carbohydrate, amino acid and vitamin biosynthesis pathways. Maternal nematode infection did not alter the vaginal or milk microbiomes. Our second hypothesis was that maternal infection would shape colonization of the neonatal microbiome. Although the pup stomach microbiome was similar to that of the maternal vaginal microbiome, pups of infected dams had higher beta-diversity at day 2, and a dramatic expansion in the abundance of Lactobacillus between days 2 and 7 compared with pups nursing uninfected dams. Our third hypothesis that maternal nematode infection altered the composition of neonatal microbiomes was confirmed as we observed up-regulation of several putatively beneficial microbial pathways associated with synthesis of essential and branched-chain amino acids, vitamins, and short-chain fatty acids. We believe this is the first study to show that a nematode living in the maternal intestine is associated with altered composition and function of the neonatal microbiome. |
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| Keywords: | 16S rRNA gene sequencing Maternal nematode infection Microbiome Mouse Nematode parasite Neonatal colonization Pregnancy |
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