A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163 |
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| Authors: | María de la Luz Mohedano Pasquale Russo Vivian de los Ríos Vittorio Capozzi Pilar Fernández de Palencia Giuseppe Spano Paloma López |
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| Affiliation: | 1.Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Calle Ramiro de Maeztu 9, Madrid 28040, Spain;2.Dipartimento di Scienze Agrarie, degli Alimenti e dell''Ambiente, University of Foggia, Via Napoli 25, Foggia 71122, Italy |
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| Abstract: | Oenococcus oeni is the main lactic acid bacterium that carries out the malolactic fermentation in virtually all red wines and in some white and sparkling wines. Oenococcus oeni possesses an array of metabolic activities that can modify the taste and aromatic properties of wine. There is, therefore, industrial interest in the proteins involved in these metabolic pathways and related transport systems of this bacterium. In this work, we report the characterization of the O. oeni ATCC BAA-1163 proteome. Total and membrane protein preparations from O. oeni were standardized and analysed by two-dimensional gel electrophoresis. Using tandem mass spectrometry, we identified 224 different spots corresponding to 152 unique proteins, which have been classified by their putative function and subjected to bioinformatics analysis. |
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| Keywords: | Oenococcus oeni proteome two-dimensional electrophoresis |
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