Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration |
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Authors: | Guilbaud Mickaël Chadeuf Gilliane Avolio Fabio François Achille Moullier Philippe Recchia Alessandra Salvetti Anna |
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Institution: | Mickaël Guilbaud, Gilliane Chadeuf, Fabio Avolio, Achille François, Philippe Moullier, Alessandra Recchia, and Anna Salvetti |
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Abstract: | The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo. |
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