首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration
Authors:Guilbaud Mickaël  Chadeuf Gilliane  Avolio Fabio  François Achille  Moullier Philippe  Recchia Alessandra  Salvetti Anna
Institution:Mickaël Guilbaud, Gilliane Chadeuf, Fabio Avolio, Achille François, Philippe Moullier, Alessandra Recchia, and Anna Salvetti
Abstract:The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号