Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity |
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| Authors: | F. Ann Ran,Patrick D. Hsu,Chie-Yu Lin,Jonathan S. Gootenberg,Silvana Konermann,Alexandro E. Trevino,David A. Scott,Azusa Inoue,Shogo Matoba,Yi Zhang,Feng Zhang |
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| Affiliation: | 1 Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA;2 McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;3 Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;4 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5 Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA;6 Harvard/MIT Division of Health Sciences and Technology, Harvard Medical School, Boston, MA 02115, USA;7 Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA;8 Program in Cellular and Molecular Medicine, Harvard Medical School, Boston, MA 02115, USA;9 Department of Genetics, Harvard Medical School, Boston, MA 02115, USA;10 Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA |
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