Transition kinetics of the conversion of blue to purple bacteriorhodopsin upon magnesium binding
Institution:
Max-Planck-Institut für Ernährungsphysiologie, D-4600 Dortmund, FRG
Abstract:
Magnesium binding to cation-depleted blue bacteriorhodopsin (b-bR) was studied spectrophotometrically as well as by following stopped-flow kinetics. There exist three kinetically different steps in the binding process, yielding purple bacteriorhodopsin (p-bR). Since only the firtst step is dependent on the concentration of the reactants, the reaction scheme
