A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase |
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| Authors: | Uchida H Morinaga H Misaki T Miyazaki T Uwajima T Obata T Endo Y Matsuda A Sasaki T |
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| Affiliation: | Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan. uchida@acbio.acbio.fukui-u.ac.jp |
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| Abstract: | By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated. |
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