Affinity chromatography techniques based on the immobilisation of peptides exhibiting specific binding activity |
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Authors: | Tozzi Cinzia Anfossi Laura Giraudi Gianfranco |
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Affiliation: | Department of Analytical Chemistry, University of Turin, Via P. Giuria 5, 10125 Turin, Italy. cinzia.tozzi@unito.it |
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Abstract: | Affinity chromatography is one of the powerful techniques in selective purification and isolation of a great number of compounds. New challenges in scientific research, such as high-throughput systems, isolation procedures that allow to obtain a single substance from a complex matrix in high degree of purity, low costs and wide availability, have led to the discovery of new tailor-made synthetic recognition systems. In this review the design, synthesis, purification and characterisation of peptides with recognition properties are discussed. Applications of peptide ligands are described and analytical tools mentioned. |
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