| 活性氧不敏感型拟南芥的突变体对H2O2的响应 |
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| 引用本文： | 何金环,李利红,宋纯鹏. 活性氧不敏感型拟南芥的突变体对H2O2的响应[J]. 植物生理学通讯, 2007, 43(1): 31-35 |
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| 作者姓名： | 何金环 李利红 宋纯鹏 |
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| 作者单位： | 郑州牧业工程高等专科学校生物工程系 郑州450011(何金环，李利红)，河南大学生命科学学院 河南开封475001(宋纯鹏) |
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| 摘 要： | 检测拟南芥ros突变株对H2O2响应的结果表明,此种突变体对H2O2有较强的耐受性,表现为气孔开度对H2O2不敏感和H2O2胁迫时的膜脂过氧化水平较低。采用激光扫描共聚焦显微术(LSCM)并结合H2O2荧光探针H2DCFDA检测外源ABA诱导保卫细胞的结果显示,突变体内荧光强度比野生型拟南芥低,暗示此种突变体消除H2O2的能力可能有提高,从而可增强植株抗氧化胁迫的能力。
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| 关 键 词： | 拟南芥 突变体 过氧化氢 激光扫描共聚焦显微术 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-12-26 |
Response of Reactive Oxygen Intermediates-insensitive Mutant Seedlings in Arabidopsis thaliana to Hydrogen Peroxide |
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| HE Jin-Huan,LI Li-Hong,SONG Chun-Peng. Response of Reactive Oxygen Intermediates-insensitive Mutant Seedlings in Arabidopsis thaliana to Hydrogen Peroxide[J]. Plant Physiology Communications, 2007, 43(1): 31-35 |
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| Authors: | HE Jin-Huan LI Li-Hong SONG Chun-Peng |
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| Abstract: | The result showed that the mutants had stronger resistance to H2O2, the stomatal aperture was not sensitive to H2O2 and had a lower level of peroxidation of membrane-lipid under the H2O2 stress. Using of the laser scanning confocal microscopy technique and combining the H2O2 fluorescence probe H2DCFDA to exam-ine the production H2O2 in guard cell by exogenous ABA, it showed that the fluorescence intensity in the mutant seedlings was lower than that in wild-type ones. It suggested that the ability of clearing H2O2 of mutant seed-lings raised, thus the resistance of seedlings to oxygen stress maybe increased. |
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| Keywords: | Arabidopsis thaliana mutant hydrogen peroxide laser scanning confocal microscopy |
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