Thidiazuron induced adventitious shoot regeneration in <Emphasis Type="Italic">Hyoscyamus niger</Emphasis> |
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Authors: | Email author" target="_blank">S?UranbeyEmail author |
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Institution: | (1) Ministry of Agriculture and Rural Affairs, Central Research Institute for Field Crops, Yenimahalle, 06110 Ankara, Turkey |
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Abstract: | A high frequency adventitious shoot regeneration protocol was developed for henbane (Hyoscyamus niger L.) using thidiazuron (TDZ). Hypocotyl, cotyledon and stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzylaminopurine and TDZ. MS medium supplemented with 16 μM TDZ was the most effective for providing 100 % regeneration frequency associated with a 19.53 shoots per hypocotyl explant. Plantlets were rooted on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid. High rooting and survival was achieved using half strength MS medium supplemented with 8 μM IBA.This study was supported by The State Planning Commission of Turkey (DPT) and University of Ankara (Project Nos.: 98K120640 and 2001K120240). |
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Keywords: | N6-benzylaminopurine henbane indole-3-butyric acid α -naphthaleneacetic acid rooting tissue culture |
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