| Abstract: | Among many aspects of plant defence responses to pathogenicinfection are changes in the composition of the exocellularmatrix. To study potential defences in white lupin (Lupinusalbus L.), suspension-cultured cells were treated for 24 h withone of three different elicitors: CuCl2, and two fungal elicitorpreparations from purified cell walls of yeast and ColletotrichumIindemuthianum. Two subsets of exocellular proteins: ionicallyboundwall proteins and proteins secreted into culture medium, wereisolated, and their patterns compared following electrophoreticseparation. Only a few proteins were observed in culture filtrateswith dominating bands at 27, 33, and 42 kDa. About 30 proteinswere observed in cell wall extracts. Changes in protein intensitiesevoked by elicitor treatments depended on the type of elicitorused, the age and composition of lupin cell culture, and concentrationof applied fungal elicitor. Based on these observations, tenproteins were chosen for N-terminal sequencing, and sequences5–30 amino acids long for nine proteins were obtained.Three of the major proteins sequenced were identified as acidicexocellular chitinase, polygalacturonaseinhibiting protein,and germin/oxalate oxidase. Key words: Lupinus aibus, defence response, exocellular proteins, elicitation, N-terminal amino acid sequencing, suspension culture |
