Biodegradation of two tetrameric lignin model compounds by a mixed bacterial culture |
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Authors: | Jouni Jokela Jukka Pellinen Mirja Salkinoja-Salonen Gösta Brunow |
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Institution: | (1) Department of General Microbiology, University of Helsinki, Mannerheimintie 172, 28 Helsinki, Finland;(2) Department of Chemistry, University of Helsinki, Vuorikatu 20, 10 Helsinki, Finland |
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Abstract: | Summary The ability of a mixed bacterial culture to decompose two tetrameric lignin model com-pounds as a sole source of carbon and
energy was investigated. The mixed bacterial culture con-sisted mainly of Gram negative rods. The tetram-ers contained two
types of lignin substructures, namely the most abundant β-O-4 ether structure in lignin and also the 5-5 biphenyl structure.
The tetramer (MW 638) containing two phe-nolic hydroxyls was decomposed readily; after 13 days of incubation, all intermediate
products formed were almost totally decomposed. The non-phenolic tetramer (MW 666) was decom-posed much more slowly; after
53 days of incuba-tion, 5% of the substrate was unchanged. When both tetramers were degraded simultaneously, the non-phenolic
tetramer was decomposed similarly to the phenolic tetramer.
Determination of molecular weights of cata-bolic products showed that the degradation of the non-phenolic tetramer had proceeded
at least to dimer level.
SKF 525A, inhibitor of cytochrome P-450, caused one catabolic product to accumulate in the culture medium. This indicates
involvement of cy-tochrome P-450 in the degradation pathway of the model compounds used.
We conclude that this mixed bacterial culture was able to degrade the lignin model compounds used and that free phenolic groups
seem to in-crease the biodegradability significantly. |
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