Molecular analysis of the human interferon-alpha gene family |
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Authors: | C Brack S Nagata N Mantei C Weissmann |
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Affiliation: | 1. Basel Institute for Immunology, 4058 Basel, Switzerland;2. Institut für Molekularbiologie I, Universität Zürich, 8093 Zürich Switzerland |
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Abstract: | Fifteen DNA clones containing sequences related to human interferon-alpha cDNA were isolated from a human chromosomal gene bank (Nagata et al., Nature 287 (1980) 401-408) and characterized by restriction mapping, R-loop and heteroduplex analysis. Nine distinct DNA segments hybridized strongly with interferon-alpha 1 cDNA and formed R-loops with poly(A) RNA from interferon-producing human leukocytes; most if not all of these segments represent functional interferon genes. Five segments hybridized weakly with the probe and did not form R-loops with the poly(A) RNA; one of these was characterized as an interferon-alpha pseudogene. Several DNA segments overlap and define a region of 36 kilobase pairs (kb) that contains three strongly and three weakly hybridizing sequences. From our data and those of Goeddel et al. (Nature 290 (1981) 20-25) we conclude that there exist at least 11 distinct genes of gene-like sequences of the interferon-alpha type in the human genome, of which most likely represents an allelic variant, and at least five pseudogenes distantly related to the interferon-alpha genes. |
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Keywords: | Recombinant DNA chromosomal DNA clones restriction enzyme analysis R-loop mapping heteroduplex analysis gene linkage bp base paires ψIFN interferon pseudogene kb kilobase pairs SDS sodium dodecyl sulfate SSC 0.15 M NaCl, 0.015 M Na · citrate, pH 7.0 |
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