Rapid isolation of nucleoli from detergent purified nuclei of various tumor and tissue culture cells |
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| Authors: | M Muramatsu Y Hayashi T Onishi M Sakai K Takai |
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| Institution: | 1. Department of Biochemistry Tokushima University School of Medicine, Tokushima, Japan;2. Laboratory of Electron Microscopy, Tokushima University School of Medicine, Tokushima, Japan |
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| Abstract: | A rapid isolation procedure of nucleoli from detergent purified nuclei of some tumor and tissue culture cells is described. The procedure makes use of a non-ionic detergent, Nonidet P40 and sodium deoxycholate to purify nuclei followed by the addition of Ca2+ or Mg2+ to strengthen the nucleoli against sonication. Enzymatically active (with respect to nucleolar RNA polymerase) nucleoli containing undegraded nucleolar RNAs may be isolated from a mouse hepatoma MH134, Ehrlich ascites tumor, HeLa cells, L cells and C3H2K cells with this procedure. |
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