Separation of t-RNAs by two-dimensional polyacrylamide gel electrophoresis |
| |
| Authors: | M Stein F Varricchio |
| |
| Institution: | Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA |
| |
| Abstract: | A pH 5.8 polyacrylamide gel electrophoresis buffer is described. Electrophoresis in this MES-citrate system at pH 5.8 separates E. coli transfer RNAs into 15 bands using 15% acrylamide gels. Polyacrylamide gel electrophoresis in a second dimension at pH 8.3 further resolves E. coli t-RNAs into 20 spots. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
