Species-specific non-expression of ribosomal RNA genes in a mammalian hybrid,the mule |
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| Authors: | Ewald Kopp Burkhard Mayr Walter Schleger |
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| Affiliation: | (1) Institute for Animal Breeding and Genetics, Linke Bahngasse 11, A-1030 Vienna, Austria;(2) Ludwig Boltzmann Institute for Immunogenetic and Cytogenetic Research, Veterinary University, Linke Bahngasse 11, A-1030 Vienna, Austria |
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| Abstract: | The expression of nucleolus organizer activity in diploid cells was investigated in a model system for mammalian hybrids, the horse-ass cross (mule), by means of sequential Ag-NOR and chromomycin A3/distamycin ADAPI (CDD) staining in lectin-stimulated peripheral blood lymphocytes (PBL). As a rule we found species-specific nonexpression of the horse-derived NOR chromosomes in the mule, whereas the ass-derived NOR chromosomes were active. The results of PBL interphase studies were compatible with the data gained from mitotic metaphase analyses. |
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