From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel |
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Authors: | Jin Zhang Ivan Khvorostov Michael Teitell |
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Affiliation: | David Geffen School of Medicine, University of California, Los Angeles |
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Abstract: | This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.Open in a separate windowClick here to view.(24M, flv) |
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