100% complete assignment of non-labile 1H, 13C, and 15N signals for calcium-loaded calbindin D9k P43G |
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| Authors: | Nur Alia Oktaviani Renee Otten Klaas Dijkstra Ruud M. Scheek Eva Thulin Mikael Akke Frans A. A. Mulder |
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| Affiliation: | (1) Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;(2) Department of Biophysical Chemistry, Lund University, PO Box 124, Lund, Sweden; |
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| Abstract: | Here we present the 100% complete assignment chemical shift of non-labile 1H, 15N and 13C nuclei of Calbindin D9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858–14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464–6465, 1994; Yamazaki et al. in Biochem 32:5656–5669, 1993b). |
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