Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis |
| |
Authors: | Guibet Marion Kervarec Nelly Génicot Sabine Chevolot Yann Helbert William |
| |
Institution: | Végétaux Marins et Biomolécules, UMR 7139 (CNRS/UPMC), Station Biologique, Roscoff, France. |
| |
Abstract: | lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan. |
| |
Keywords: | λ-Carrageenan Carrageenase Gigartina skottsbergii Hybrid structure |
本文献已被 ScienceDirect PubMed 等数据库收录! |