The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity |
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Authors: | Sun Tae Kim Seok Yu Young Hyun Kang Sang Gon Kim Jae-Yean Kim Sun-Hyung Kim Kyu Young Kang |
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Institution: | (1) Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Korea;(2) Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Korea;(3) Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, 660-701, Korea;(4) Department of Environmental Horticulture, University of Seoul, Seoul, 130-743, Korea |
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Abstract: | We previously reported that rice blast fungus or jasmonic acid induced the expression of rice pathogenesis-related class 10
(JIOsPR10) proteins (Kim et al. 2003, 2004). However, no further studies have been carried out to examine the expression, localization, and enzymatic activity of this
protein in either developmental tissues or in tissues under abiotic stress conditions. In this study, rice JIOsPR10 was examined
by Western blot analysis, immunolocalization, and biochemical assays. Western blots revealed that the JIOsPR10 protein was
expressed in developmental tissues, including in flower and root. The protein was also expressed under abiotic stresses, such
as occurs during senescence and wounding. Using immunohistochemical techniques, we determined that expression of JIOsPR10
was localized to the palea of flower, in the exodermis, and inner part of the endodermis of the root. In senescencing tissues
of leaf and coleoptiles, its expression was localized in vascular bundles. The RNase activity using JIOsPR10 recombinant protein
was determined and abolished after treatment with DTT in a native in-gel assay. To test this, we created JIOsPR10 mutant proteins
containing serine substitutions of amino acids C81S, C83S, or both and examined their RNase activities. The activity of the
C83S mutant was decreased in the agarose gel assay compared to the wild type. Taken together, we hypothesize that the JIOsPR10
protein possesses RNase activity that is sensitive to DTT, suggesting the importance of the disulfide bonding between cysteine
residues and that it might play a role in constitutive self-defense mechanisms in plants against biotic and abiotic stresses. |
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Keywords: | Abiotic stresses Biotic stresses Pathogenesis-related (PR) proteins RNase activity |
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