The effect of IgE-mediated mast cell degranulation on the expression of experimental contact sensitivity in the mouse |
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| Authors: | Y A Mekori J C Chang |
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| Affiliation: | 1. Allergy-Immunology Unit, Meir General Hospital, Kfar-Saba, 44281 Israel;2. Sackler School of Medicine, Tel-Aviv University, Israel;3. Department of Microbiology and Immunology, University of Colorado School of Medicine, Denver, Colorado 80262 USA;1. Department of Biomedical Sciences, School of Biological and Environmental Sciences, Kwansei Gakuin University, 1 Gakuen Uegahara, Sanda, Hyogo, 669-1337, Japan;2. Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI, 48109-2216, USA;3. Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Anschutz Medical Campus, 12800 East 19th Avenue, Aurora, CO, 80045, USA;4. Department of Morphological Biology, Ohu University School of Dentistry, 31-1 Misumido Tomitamachi, Koriyama, Fukushima, 963-8611, Japan;1. Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan;1. Department of Dermatology, Radboud University Medical Center (Radboudumc), Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen, The Netherlands;2. Department of Molecular Developmental Biology, Faculty of Science, Radboud University, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen, The Netherlands;3. Department of Human Genetics, Radboud University Medical Center (Radboudumc), Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen, The Netherlands;1. Infection, Immunity, Inflammation, and Physiological Medicine Programme, Immunobiology Section, University College London Institute of Child Health, London, United Kingdom;2. Department of Chemistry, University College London, London, United Kingdom;3. Dermatopharmacology, Sir Henry Wellcome Laboratories, Faculty of Medicine, University of Southampton, Southampton General Hospital, Southampton, United Kingdom;4. Dermatology, University Hospital Southampton National Health Service Foundation Trust, Southampton, United Kingdom;1. Cutaneous Biology Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA;2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People’s Republic of China;3. School of Pharmacy, University of Wisconsin-Madison, Wisconsin, USA;1. Department of Dermatology, Severance Hospital, Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea;2. Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea;3. Department of Dermatology, Yanbian University Hospital, Yanji, Jilin, China;4. Department of Plastic and Reconstructive Surgery, Severance Hospital, Institute for Human Tissue Restoration, Yonsei University College of Medicine, Seoul, Korea |
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| Abstract: | The effects of mast cell activation/degranulation on the elicitation of contact sensitivity (CS) to oxazolone and dinitrofluorobenzene were investigated. Mice were actively sensitized to oxazolone by epicutaneous painting followed by ear challenge. Passive sensitization to DNFB was induced by intradermal injections of dinitrophenol (DNP)-specific cloned T cells in the ears. Mast cells in the challenged ears were activated in various time periods by inducing a passive cutaneous anaphylaxis reaction where passive sensitization with monoclonal IgE anti-DNP antibodies was followed by iv injection of DNP-BSA. This combination of immediate and delayed-type hypersensitivity reactions resulted in a significant increase of ear swelling without any noticeable effect on cellular infiltration when the contact response was evaluated a short time (3-4 hr) after mast cell activation. The very same results were obtained in naive (unsensitized) mice, indicating that this reaction was nonspecific. However, when the CS reaction was evaluated at its peak, i.e., 24 hr post challenge, mast cell activation that had been induced 0.5-11 hr after ear challenge did not have any significant effect on both swelling and cellular infiltration when the latter was evaluated by a radiometric assay. We conclude that in these systems mast cell activation/degranulation makes little or no contribution to the modulation of T-cell activity. |
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