Human somatotropin: semisynthesis of the hormone by noncovalent interaction of the NH2-terminal fragment with synthetic analogs of the COOH-terminal fragment |
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Authors: | C H Li J Blake T Hayashida |
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Institution: | 1. Hormone Research Laboratory University of California San Francisco, California 94143 USA;2. Department of Anatomy University of California San Francisco, California 94143 USA |
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Abstract: | Complementation of the natural NH2-terminal fragment (consisting of 134 amino acid residues) with synthetic analogs of COOH-terminal fragments of 52 or 47 amino acids of reduced-carbamidomethylated human somatotropin gave recombinants with full growth-promoting activity as evidenced by the tibia test. Radioimmunoassay data show that the semisynthetic recombinant hormones possess nearly full immunoreactivity as compared to the native molecule. |
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