基于响应面法优化匍枝根霉TP-02液态发酵纤维素酶条件

利用Plackett-Burman设计法(Plackett-Burman,PB),对影响根霉TP-02液态发酵产纤维素酶的8个因子进行了筛选,结果表明,影响该菌发酵产纤维素酶的主要因子为麸皮与稻草的比例、槐糖、Tween 80。利用最陡爬坡试验逼近最大响应区域,在此基础上,采用响应面法(ResponseSurface Methodology,RSM)对这3个因子的影响进行研究,得出纤维素酶产量的数学模型,通过对二次多项回归方程求解,得到3个因子的最优用量:麸皮稻草比例为:3.7:1,槐糖量为:0.62%,Tween 80为0.68 g/L,在优化后的条件下培养96 h,纤维素酶滤纸酶活可达到8.13 IU/mL比优化前提高了38.97%。

Optimization of culture conditions for production of cellulase by liquid fermentation of Rhizopus stolonifer TP-02

The Plackett-Burman de,sign was used to evaluate the effects of the eight factors on the production of celluase by liquid fermentation of Rhizopus. TP-02. The significant ones among the eight factors were determined as follows： proportion of bran and straw, sophorose, Tween 80. Then, the steepest ascent path was used to approach the optimal region of eulture condition. At last, the response surface methodology （RSM） was used to optimize the three factors. By solving the quadratic regression model equation, the optimal conditions of these variables were determined as： proportion of bran and straw 3.7：1, sophorose 0.62%, Tween 80 0.68g/L. After cultured under the optimized condition for 96 h, the filter paper activity of cellulase reached 8.69 IU/mL, increased by 48.5 % than that of the former.