Plasmid-encoded lysostaphin endopeptidase gene of Staphylococcus simulans biovar staphylolyticus |
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Authors: | Lucie S Heath HE Heath Gary L Sloan |
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Institution: | Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa 228, Japan |
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Abstract: | A 12.2-kilobase (kb) BclI fragment containing the lysostaphin endopeptidase gene was cloned from Staphylococcus simulans biovar staphylolyticus into Escherichia coli. The gene was expressed in E. coli and the gene product apparently was secreted into the periplasmic space. The gene was localized to a 3.3-kb region of the cloned fragment and this region was shown to contain a staphylococcal promoter for the endopeptidase gene. By hybridization analysis, the endopeptidase gene was shown to reside on the largest of five plasmids in S. simulans biovar staphylolyticus. No additional copies of this gene were detected in the genome. |
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Keywords: | Sendai virus HN glycoprotein Haemagglutinin Neuraminidase (Antigenic determinant Monoclonal antibody) |
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