Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors |
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Authors: | Noboru Sato Kenji Matsuda Chie Sakuma Douglas N. Foster Ronald W. Oppenheim Hiroyuki Yaginuma |
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Affiliation: | Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. nsato@fmu.ac.jp |
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Abstract: | Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo. |
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