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Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors
Authors:Noboru Sato   Kenji Matsuda   Chie Sakuma   Douglas N. Foster   Ronald W. Oppenheim     Hiroyuki Yaginuma
Affiliation:Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. nsato@fmu.ac.jp
Abstract:Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo.
Keywords:
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