The broad diversity of neutral and sialylated oligosaccharides derived from human salivary mucins. |
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Authors: | A Klein C Carnoy J M Wieruszeski G Strecker A M Strang H van Halbeek P Roussel G Lamblin |
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Institution: | Unité des Protéines, Institut National de la Santé et de la Recherche Médicale No. 16, Lille, France. |
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Abstract: | Mucin glycopeptides were prepared from the salivary mucins of 20 healthy donors with blood group O. The carbohydrate chains of the high-molecular-weight mucins were released by alkaline borohydride treatment. Neutral and monosialylated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and HPLC. The structures of the oligosaccharide-alditols were determined by high-resolution 1H-NMR spectroscopy in combination with fast atom bombardment mass spectrometry and methylation analysis. Thirty-seven oligosaccharide-alditols were characterized and illustrate the extreme diversity of the salivary mucins carbohydrate chains. This diversity might represent a mosaic of bacterial adhesion sites and be involved in the early events of the nonimmune defense of the oral cavity. Among these 37 oligosaccharide-alditols, 31 have not been previously described in human saliva and five of these are novel structures: formula: see text] |
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