Arabidopsis DOK1 encodes a functional dolichol kinase involved in reproduction |
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Authors: | Kazue Kanehara Yueh Cho Ying‐Chen Lin Chia‐En Chen Chao‐Yuan Yu Yuki Nakamura |
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Institution: | 1. Academia Sinica, Institute of Plant and Microbial Biology, Nankang Taipei, Taiwan;2. Molecular and Biological Agricultural Sciences Program, Academia Sinica, Taiwan International Graduate Program, Taipei, Taiwan;3. Biotechnology Center, National Chung‐Hsing University, Taichung, Taiwan;4. Department of Applied Science and Engineering, Muroran Institute of Technology, Muroran, Hokkaido, Japan;5. Graduate Institute of Biotechnology, National Chung‐Hsing University, Taichung, Taiwan |
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Abstract: | Dolichol phosphate (Dol‐P) serves as a carrier of complex polysaccharides during protein glycosylation. Dol‐P is synthesized by the phosphorylation of dolichol or the monodephosphorylation of dolichol pyrophosphate (Dol‐PP); however, the enzymes that catalyze these reactions remain unidentified in Arabidopsis thaliana. We performed a genome‐wide search for cytidylyltransferase motif‐containing proteins in Arabidopsis, and found that At3g45040 encodes a protein homologous with Sec59p, a dolichol kinase (DOK) in Saccharomyces cerevisiae. At3g45040, designated AtDOK1, complemented defects in the growth and N‐linked glycosylation of the S. cerevisiae sec59 mutant, suggesting that AtDOK1 encodes a functional DOK. To characterize the physiological roles of AtDOK1 in planta, we isolated two independent lines of T‐DNA‐tagged AtDOK1 mutants, dok1‐1 and dok1‐2. The heterozygous plants showed developmental defects in male and female gametophytes, including an aberrant pollen structure, low pollen viability, and short siliques. Additionally, the mutations had incomplete penetrance. These results suggest that AtDOK1 is a functional DOK required for reproductive processes in Arabidopsis. |
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Keywords: |
Arabidopsis thaliana
dolichol kinase reproduction cytidylyltransferase protein glycosylation N‐linked glycosylation |
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