Conversion of gamma-butyrobetaine to L-carnitine by Achromobacter cycloclast |
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Authors: | Naidu G S Lee I Y Cho O K Park Y H |
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Institution: | (1) Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, South Korea, KR |
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Abstract: | L -Carnitine is an ubiquitous substance that plays a major role in the transportation of long-chain fatty acids. We investigated
crucial factors that influence microbial conversion of γ-butyrobetaine to L-carnitine using an Achromobacter cycloclast strain. Two-stage culture results showed that γ-butyrobetaine induced enzymes essential for the conversion, which suggests
that the precursor should be present in the initial cell growth stage. The addition of yeast extract enhanced L-carnitine production whereas inorganic nitrogen sources inhibited it. Under nitrogen-limiting conditions, the cells accumulated
poly-β-hydroxybutyrate instead of L-carnitine. Among the trace elements tested, nickel addition enhanced L-carnitine production by almost twice that of the control and copper strongly inhibited the conversion. L-Carnitine production was reduced when the medium contained inorganic salts of sodium, potassium, and calcium at a concentration
greater than 2 g l−1. A higher L-carnitine yield was achieved when cells were incubated in a lower culture volume. The optimal pH for L-carnitine production was 5 to 5.5, whereas that of growth was 7.0, indicating that a pH shift was required. Under optimal
conditions, L-carnitine concentrations as high as 15 g l−1 were obtained in 62 h with a 45% molar conversion yield. Journal of Industrial Microbiology & Biotechnology (2001) 26, 309–315.
Received 26 November 2000/ Accepted in revised form 27 February 2001 |
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Keywords: | : L-Carnitine γ -Butyrobetaine production Achromobacter cycloclast |
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