Haemopoietic Inductive Capacity of Irradiated Stromal Cell Layers In Human Micro Long-Term Bone Marrow Cultures |
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| Authors: | P Brühl H-G Mergenthaler P Dörmer |
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| Institution: | Gesellschaft für Strahlen- und Umweltforschung, Instiiut für Experimentelle Hämatologie, München and Medizinische Klinik III, Klinikum Groβhadern der Ludwig-Maximilians-Universität Münchem, F. R. G. |
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| Abstract: | Abstract. In a micro long-term bone marrow culture (LTBMC) system the effects of irradiation on confluent stromal cell layers were studied. In order to individually analyse the number of granulocyte-macrophage colony-forming cells (GM-CFC) per LTBMC a miniaturized human GM-CFC assay was established. the normalized GM-CFC numbers in the micro-assay compared well with data by the conventional GM-CFC assay. Pre-formed stromal cell layers were irradiated with doses up to 20 Gy and subsequently recharged with allogeneic bone marrow cells (BMC). Immediately before recharge the BMC were stromal cell-depleted by nylon wool filtration. When stromal cell-depleted BMC were inoculated on empty culture dishes, in vitro haemopoiesis rapidly declined. Sustained GM-CFC production, however, was seen when these cells were used as a second inoculum. It is concluded that irradiation doses of up to 20 Gy do not cause alteration of the haemopoietic inductive capacity of confluent stromal cell layers. |
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