Enhancement in the viability and biosensing activity of freeze-dried recombinant bioluminescent bacteria |
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Authors: | Sue Hyung Choi Man Bock Gu |
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Affiliation: | (1) Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), 1 Oryong-dong, Puk-gu, 500-712 Kwangju, Korea |
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Abstract: | The genetically-engineeredEscherichia coli strain, DPD2540, which contains afabA::luxCDABE fusion gene, gives a bioluminescent output when membrane fatty acid synthesis is needed. For more practical application of this strain in the field as biosensor, freeze-drying was adopted. A 12% sucrose solution with Luria-Bertani (LB) broth, as determined by the viability after freeze-drying, was found to be the most effective composition for lyophilization solution among various compositions tested. Rapid freezing with liquid nitrogen also gave the best viability after freeze-drying as compared to samples frozen at −70°C and −20°C. The biosensing activities of the cells showed a greater sensitivity when the cells from the exponential phase were freeze-dried. Finally, the optimum temperature for use of the freeze-dried cells in the biosensor field was determined. |
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Keywords: | recombinant bioluminescent bacteria bioluminescence cryoprotectant optimum freezing and monitoring temperatures |
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