| 鸡骨骼肌卫星细胞的分离培养、鉴定及生物学特性研究 |
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| 引用本文： | 陈岩,王琨,朱大海.鸡骨骼肌卫星细胞的分离培养、鉴定及生物学特性研究[J].遗传,2006,28(3):257-260. |
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| 作者姓名： | 陈岩 王琨 朱大海 |
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| 作者单位： | 1．哈尔滨工业大学分子与细胞发育生物学实验室，哈尔滨150001；2.中国协和医科大学医学分子生物学国家重点实验室，北京100005 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973计划)，国家科技攻关项目 |
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| 摘 要： | 采用I型胶原酶和胰蛋白酶二步消化，经体外培养获得鸡骨骼肌卫星细胞，并通过检测肌卫星细胞特异基因的表达进行鉴定。结果表明：二步消化法适用于鸡骨骼肌卫星细胞的分离和获取，此方法分离得到的鸡骨骼肌卫星细胞表达卫星细胞特异的标志基因desmin和Pax7，并具有良好的增殖和分化能力，为鸡骨骼肌细胞增殖、分化和再生机制的研究提供技术平台。
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| 关 键 词： | 鸡 骨骼肌 肌肉发生 卫星细胞 |
| 文章编号： | 0253-9772(2006)03-0257-04 |
| 收稿时间： | 2005-07-29 |
| 修稿时间： | 2005-10-28 |
Isolation, Culture, Identification and Biological Characteristics of Chicken Skeletal Muscle Satellite Cells |
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| CHEN Yan,WANG Kun,ZHU Da-Hai.Isolation, Culture, Identification and Biological Characteristics of Chicken Skeletal Muscle Satellite Cells[J].Hereditas,2006,28(3):257-260. |
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| Authors: | CHEN Yan WANG Kun ZHU Da-Hai |
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| Institution: | 1. Molecular and Cellular Developmental Biology Laboratory, Harbin Institute of Technology, Harbin 150001,China; 2. National Laboratory of Medical Molecular Biology, Department of Biochemistry, Peking Union Medical College, Beijing 100005, China |
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| Abstract: | Chicken skeletal muscle satellite cells were obtained by a two-step method of collagenase-I and trypsin digestion, followed by in vitro culture. They were further identified by specific gene expression assays. In addition, cell biological characteristics and myogenic genes expression were analyzed. Results showed that satellite cells from chicken skeletal muscle tissue expressed pax7, desmin, and possessed ability to proliferate and differentiate. The technical platform was established to study the mechanism of myogenic proliferation, differentiation and muscle regeneration. |
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| Keywords: | chicken skeletal muscle satellite cells myogenesis |
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