Effect of dissociative methods on cortisol binding and glutamyltransferase inducibility in chick embryo retina |
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Authors: | Joel B. Piperberg Liane Reif-Lehrer |
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Affiliation: | (1) Department of Retina Research, Eye Research Institute of Retina Foundation, 02114 Boston, MA;(2) Department of Ophthalmology, Harvard Medical School, 02114 Boston, MA |
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Abstract: | The ability of the isolated embryonic chick retina (12 day) to bind a steroid (cortisol) decreases when the tissue is dissociated; the extent of this decrease depends upon the method of dissociation. Trypsin and mechanical dissociation decreased cortisol binding slightly; papain dissociation essentially eliminated it. Cortisol binding decreased with time in culture in both whole retina and monolayer cultures; this decrease may reflect, in part, a similar developmental decreasein ovo. Inducibility of glutamine synthetase in whole retinas and retinal monolayers prepared with either trypsin or papain also decreased with time in culture. For whole and trypsin-dissociated retinas, the drop in inducibility correlates with a drop in cortisol-binding capacity. This was not the case for monolayer cultures prepared by papain dissociation. |
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Keywords: | Retina tissue dissociation cell cultures chick embryo glutamine synthetase induction cortisol papain trypsin acetyltrypsin mechanical dissociation cortisol binding |
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