Sequence analysis of the gene encoding H antigen in <Emphasis Type="Italic">Escherichia coli</Emphasis> isolated from food in Morocco |
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| Authors: | Samira Badri Aziz Fassouane Ingrid Filliol Mohammed Hassar Nozha Cohen |
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| Institution: | (1) Department of Microbiology, Mechnikov Research Institute for Vaccines and Sera of The Russian Academy of Medical Sciences, 5-a Pereulok Maly Kazenny, 105064 Moscow, Russia;(2) Bacteriology Unit, National Institute for Health and Welfare (THL), P.O. Box 30, 00271 Helsinki, Finland;(3) TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, People’s Republic of China;(4) Present address: College of Biological Sciences and Biotechnology, Shenyang Agricultural University, 110161 Shenyang, People’s Republic of China |
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| Abstract: | In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding
(fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies. |
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