Sequences essential for IS50 transposition. The first base-pair |
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Authors: | C Sasakawa S H Phadnis G F Carle D E Berg |
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Institution: | Department of Microbiology and Immunology Box 8093, Washington University School of Medicine St Louis, Miss. 63110, U.S.A. |
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Abstract: | Sequences near the ends of the insertion element IS50 are essential for its transposition, probably because they serve as sites upon which the IS50-encoded transposase protein acts. To determine if these essential sequences include the first base-pair at each end of IS50 we generated 5'C to 5'G transversions at these positions. Each mutation reduced the transposition frequency to 1% to 2% of wild-type. DNA sequence analyses showed that the mutant 5'G is preserved during transposition. |
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