Soluble CD40 ligand-activated B cells from patients with chronic hepatitis B virus infection as antigen presenting cells to induce hepatitis B virus specific cytotoxic T lymphocytes |
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| Authors: | Yong Liu Rui Huang Yali Xiong Qi Zhao Guangmei Chen Juan Xia Chao Wu |
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| Affiliation: | 1. Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China;2. Department of Infectious Diseases, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China;3. Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China;4. Department of Infectious Diseases, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210008, China |
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| Abstract: | Chronic hepatitis B virus (HBV) infection is the result of an inadequate antiviral immune response to the virus. In this study, we aimed to investigate whether the soluble CD40 ligand-activated B (CD40-B) cells could present antigen and induce specific cytotoxic T lymphocytes (CTLs) in patients with chronic HBV infection. We observed that after activated by sCD40L, the expression of CD80, CD86, major histocompatibility complex (MHC) I and II molecules on the CD40-B cells was significantly increased. Cytometry and fluorescence microscopy showed that more than 41.34% CD40-B cells were loaded by the HBcAg peptide. Furthermore, after been activated and HBcAg18–27 antigen peptide pulsed, B cells obtained from patients with chronic HBV infection could induce HBcAg18–27 specific CTLs in vitro. Taken together, our results show that B cells from patients with chronic HBV infection can be activated by sCD40L and may function as antigen presenting cells and induce HBV-specific CTLs. |
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| Keywords: | APCs, antigen presenting cells CD40L, CD40 ligand CD40-B, CD40L-activated B CHB, chronic hepatitis B CTLs, cytotoxic T lymphocytes DCs, dendritic cells HBcAg, hepatitis B core antigen HBsAg, hepatitis B surface antigen HBV, hepatitis B virus HCC, hepatocellular carcinoma MHC, major histocompatibility complex PBMCs, peripheral blood mononuclear cells sCD40L, soluble CD40 ligand |
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