Mining secreted proteins that function in pepper fruit development and ripening using a yeast secretion trap (YST) |
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Authors: | Je Min Lee Sang-Jik Lee Jocelyn K.C. Rose Inhwa Yeam Byung-Dong Kim |
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Affiliation: | 1. Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea;2. Department of Horticultural Science, Kyungpook National University, Daegu, Republic of Korea;3. Biotechnology Institute, Nongwoo Bio Co, Ltd, Yeoju, Republic of Korea;4. Department of Plant Biology, Cornell University, Ithaca, NY, USA;5. Department of Horticulture and Breeding, Andong National University, Andong, Republic of Korea |
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Abstract: | Plant cells secrete diverse sets of constitutively- and conditionally-expressed proteins under various environmental and developmental states. Secreted protein populations, or secretomes have multiple functions, including defense responses, signaling, metabolic processes, and developmental regulation. To identify genes encoding secreted proteins that function in fruit development and ripening, a yeast secretion trap (YST) screen was employed using pepper (Capsicum annuum) fruit cDNAs. The YST screen revealed 80 pepper fruit-related genes (CaPFRs) encoding secreted proteins including cell wall proteins, several of which have not been previously described. Transient GFP-fusion assay and an in planta secretion trap were used to validate the secretion of proteins encoded by selected YST clones. In addition, RNA gel blot analyses provided further insights into their expression and regulation during fruit development and ripening. Integrating our data, we conclude that the YST provides a valuable functional genomics tool for the identification of substantial numbers of novel secreted plant proteins that are associated with biological processes, including fruit development and ripening. |
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Keywords: | Secretome Capsicum Yeast secretion trap Fruit ripening |
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